References
References
DiGiuseppe, M., Haberer, S., Salciccioli, K., Sanader, M., Vavitas, A. (2012). Nelson Education, Biology text book Grade 12
Nobelprize.org. Nobel Media AB (2014),The Discovery of Insulin". Retrieved from the URL; <http://www.nobelprize.org/educational/medicine/insulin/discovery-insulin.html>
Erika Gebel (2013). Making Insulin, behind-the-scenes look at producing a life saving medication
Freeman W. H. (2000). Making a Recombinant DNA, National centre for Biotechnology, US, National library for medicine
Cohen, S. N., A. C. Y. Chang, H. W. Boyer, and R. B. Helling. (1973). Inserting DNA into the plasmid ceel, Construction of biologically functional bacterial plasmids in vitro. Proceedings of the National Academy of Sciences 70: 3240–3244.
DNA Cloning. Michael Andrew Quail, The Wellcome Trust Sanger Institute, Cambridge, UK. Published online: December 2010
DOI: 10.1002/9780470015902.a0005344.pub2
Source: Boundless. “Bacterial Transformation.” Boundless Microbiology. Boundless, 21 Jul. 2015. Retrieved 19 Mar. 2016 from https://www.boundless.com/microbiology/textbooks/boundless-microbiology-textbook/microbial-genetics-7/genetic-transfer-in-prokaryotes-81/bacterial-transformation-442-6842/
The history of producing Insulin gene from animals by Dr. Frederic Banting, retrieved from the URL;
https://www.youtube.com/watch?v=O8FriHOjsts
The production of Insulin gene using Genetic engineering, retrieved from the URL;
https://www.youtube.com/watch?v=iMosKBs-v0E
How to make a Recombinant DNA using two fragments from two different sources animation video, retrieved from the URL;
https://www.youtube.com/watch?v=YdjvUv-1vCI
Inserting DNA into bacterial cell, retrieved from the URL;
https://www.youtube.com/watch?v=MIfDx417SDs
Separating the DNA molecules into fragments based on their size and charge using Gel Electrophoresis, retrieved from the URL;
https://www.youtube.com/watch?v=vq759wKCCUQ